MAB3580

antibody from Novus Biologicals

Targeting: FKBP8 FKBP38, FKBPr38

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3580

Provider

Novus Biologicals

Product name

Mouse Monoclonal FKBP38 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects endogenous human, mouse and rat FKBP38 in Western blots.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Phosphatidic acid activates mammalian target of rapamycin complex 1 (mTORC1) kinase by displacing FK506 binding protein 38 (FKBP38) and exerting an allosteric effect.

Yoon MS, Sun Y, Arauz E, Jiang Y, Chen J
The Journal of biological chemistry 2011 Aug 26;286(34):29568-74

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat FKBP38 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat FKBP38 Monoclonal Antibody (Catalog # MAB3580) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FKBP38 at approximately 60 to 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.