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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- MAB11794 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal AGER Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse RAGE in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant rat RAGE, 20% - 50% cross-reactivity with recombinant human RAGE, and no cross-reactivity with recombinant canine RAGE is observed.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- RAGE in Mouse Spinal Cord. RAGE was detected in perfusion fixed frozen sections of mouse spinal cord using Rat Anti-Mouse RAGE Monoclonal Antibody (Catalog # MAB11794) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to the plasma membranes of motor neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
