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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- FAB11795G-100UG - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal AGER Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse RAGE in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant canine RAGE and no cross-reactivity with recombinant human RAGE or recombinant rat RAGE is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 0.2 mg/ml
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of RAGE in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) unstimulated or (B) stimulated to induce Th1 cells were stained with Rat Anti-Mouse RAGE Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB11795G) and Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A). Quadrant markers were set based on control antibody staining (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.
