NBP2-36552

antibody from Novus Biologicals

Targeting: TBC1D15 DKFZp761D0223, FLJ12085

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-36552

Provider

Novus Biologicals

Product name

Rabbit Polyclonal TBC1D15 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TBC1D15 Antibody [NBP2-36552] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBC1D15 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: TBC1D15 Antibody [NBP2-36552] - Detection of human TBC1D15 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBC1D15 antibody NBP2-36552 used for IP at 6 ug per reaction. TBC1D15 was also immunoprecipitated by rabbit anti-TBC1D15 antibody NBP2-36553. For blotting immunoprecipitated TBC1D15, NBP2-36552 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.