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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-75922 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal VPS26A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: VPS26 Antibody [NBP2-75922] - Total protein from A431, HeLa, and HepG2 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-VPS26A in 2.5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: VPS26 Antibody [NBP2-75922] - IHC analysis of a formalin fixed paraffin embedded tissue section of the mouse testis using 1:350 dilution of VPS26A antibody (NBP2-75922). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.
