PA1-972-A647

antibody from Invitrogen Antibodies

Targeting: PSMB8 beta5i, D6S216E, LMP7, PSMB5i, RING10

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-972-A647

Provider

Invitrogen Antibodies

Product name

PSMB8 Polyclonal Antibody, Alexa Fluor™ 647

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1972A647 detects proteasome 20S LMP7 from human cells.

Conjugate

Red dye

Concentration

1 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Proteasome 20S LMP7 (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Proteasome 20S LMP7 Polyclonal Antibody, AlexaFluor 647 conjugate (Product # PA1-972-A647) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Conjugate

Red dye