MA5-31515

antibody from Invitrogen Antibodies

Targeting: USP7 HAUSP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-31515

Provider

Invitrogen Antibodies

Product name

USP7 Monoclonal Antibody (GT481)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Keep as concentrated solution.

Antibody clone number

GT481

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of USP7 in A) 293T whole cell extract, B) A431 whole cell extract, C) HeLa whole cell extract, D) HepG2 whole cell extract using USP7 monoclonal antibody (Product # MA5-31515) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of USP7 in A) 293T whole cell extract, B) A431 whole cell extract, C) HeLa whole cell extract, D) HepG2 whole cell extract using USP7 monoclonal antibody (Product # MA5-31515) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using USP7 Monoclonal Antibody (GT481) (Product # MA5-31515). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with USP7 Monoclonal Antibody (GT481) (Product # MA5-31515) diluted at 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

USP7 Monoclonal Antibody (GT481) detects USP7 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: USP7 protein stained by USP7 Monoclonal Antibody (GT481) (Product # MA5-31515) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of USP7 in HepG2 whole cell extracts with USP7 monoclonal antibody (Product # MA5-31515) using 5 µg of sample. Sample was then incubated with anti-mouse IgG secondary antibody.