Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-11194 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), A549 (Lane 3), MCF7 (Lane 4), HT-29 (Lane 5), HEK-293 (Lane 6), KARPAS 299 (Lane 7), Hep G2 (Lane 8), Jurkat (Lane 9) and COS-7 (Lane 10). The blot was probed with Anti-SAE2 Polyclonal Antibody (Product # PA5-11194, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 71 kDa band corresponding to SAE2 was observed across the cell lines tested. An additional non specific band was observed at 58 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of UBA2/SAE2 using a UBA2/SAE2 polyclonal antibody (Product # PA5-11194) in Jurkat lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SAE2 was achieved by transfecting HeLa cells with SAE2 specific siRNAs (Silencer® select Product # s19547, s19548). Western blot analysis (Fig. a) was performed using whole cell extracts from the SAE2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SAE2 Polyclonal Antibody (Product # PA5-11194, 1:2000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SAE2.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a UBA2/SAE2 polyclonal antibody (Product # PA5-11194), followed by HRP-conjugated secondary antibody and AEC staining.