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- Antibody Data
- Antigen structure
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- Validations
- ELISA [1]
- Other assay [2]
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- Product number
- 69020-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDGF-BB Monoclonal Antibody (4G11C7), NeutraKine®
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Reconstitute with water to the desired concentration.
- Antibody clone number
- 4G11C7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Indirect ELISA was carried out by coating recombinant Human PDGF bb (Cat.NO. HZ-1308) at 70 ng/well followed by blocking and adding serial diluted PDGF bb antibody 69020-1-Ig and 69520-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Recombinant human PDGF bb (Cat.NO. HZ-1308) stimulates the proliferation of BALB/3T3 cells (mouse fibroblast cell) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human PDGF bb (20 ng/mL HZ-1308) is neutralized by mouse anti-human PDGF bb monoclonal antibody 69020-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 20-50 μg/mL. The NeutraControl mouse anti-human PDGF bb monoclonal antibody 69520-1-Ig could recognize human PDGF bb, but could not neutralize human PDGF bb at the range of 3.125-200 μg/mL(green curve, refer to top X-right Y axis).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Recombinant human PDGFbb (Cat.NO. HZ-1308) stimulates proliferation of BALB/3T3 (mouse fibroblast cell line) in a dose dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human PDGFbb (20 ng/mL HZ-1308) is neutralized by mouse anti-human PDGFbb monoclonal antibody 69020-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 20-50 μg/mL.
