PA5-52298

antibody from Invitrogen Antibodies

Targeting: ECD GCR2, hSGT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52298

Provider

Invitrogen Antibodies

Product name

ECD Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: DEDFECLDSD DDLDFETHEP GEEASLKGTL DNLKSYMAQM DQELAHTCIS KSFTTRNQVE PVSQTTDNNS DEEDSGTGES VMAPVDVDLN LVSNILESYS SQAGLAGPAS NLLQSMGVQL PDNTDHRPTS KP

Concentration

0.08 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ECD in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using an ECD Polyclonal Antibody (Product # PA5-52298).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of ECD in human cell line A-431 shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using an ECD Polyclonal Antibody (Product # PA5-52298).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ECD in human colon using ECD Polyclonal Antibody (Product # PA5-52298) shows moderate to strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ECD in human endometrium using ECD Polyclonal Antibody (Product # PA5-52298) shows weak to moderate nuclear positivity in stromal cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ECD in human testis using ECD Polyclonal Antibody (Product # PA5-52298) shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ECD in human duodenum using ECD Polyclonal Antibody (Product # PA5-52298) shows moderate to strong nuclear positivity in glandular cells.