711656

antibody from Invitrogen Antibodies

Targeting: GABBR2 GABABR2, GPR51, GPRC3B, HG20

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

711656

Provider

Invitrogen Antibodies

Product name

GABBR2 Recombinant Polyclonal Antibody (19HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Mouse, Rat, Dog.

Antibody clone number

19HCLC

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK 293 cells stably transfected with GABA B Receptor 2 (GABABR2) were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads. Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. Membranes were then incubated with Anti-GABABR2 Recombinant Rabbit Polyclonal Antibody (Product # 711656 at a concentration of 1 µg/mL). Broad Bands migrating 65 and 130 kDa corresponding to GABABR2 monomers and dimers, respectively, were observed.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK 293 cells stably transfected with GABA B Receptor 2 (GABABR2) were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads. Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. Membranes were then incubated with Anti-GABABR2 Recombinant Rabbit Polyclonal Antibody (Product # 711656 at a concentration of 1 µg/mL). Broad Bands migrating 65 and 130 kDa corresponding to GABABR2 monomers and dimers, respectively, were observed.