NBP2-75197

antibody from Novus Biologicals

Targeting: ACKR1 CCBP1, CD234, DARC, Dfy, FY, GPD

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

NBP2-75197

Provider

Novus Biologicals

Product name

Rabbit Monoclonal DARC Antibody

Antibody type

Monoclonal

Description

Protein A purified. The antibody binds specifcally to the pentapeptide FEDVW of the Duffy receptor.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.2 mg

Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DARC Antibody (2C3) [NBP2-75197] - Western Blot using anti-DARC antibody 2C3 (Ab00893) Human liver lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 2C3 (NBP2-75197) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for DARC is 35.6kDa. NBP2-75197 successfully detected DARC in human liver lysate.