Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- AP32843PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti mGluR5 / GRM5
- Antibody type
- Polyclonal
- Antigen
- Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 5 gene product, but are shared between the Human (NP_001137303.1, NCBI) and Mouse (Q3UVX5.2, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
- Reactivity
- Human, Mouse
- Host
- Chicken/Avian
- Vial size
- 1.0 ml
- Concentration
- 0.3 mg/ml of anti-peptide antibody mixture (based on Bradford assay readings using BSA as a standard)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- mGluR5Â staining of pyramidal and granule cell neurons in the hippocampal formation of an adult transgenic Mouse brain (red satining). Green staining is GFP-immunoreactivity under the control of a constitutive actin promoter