Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF6138 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal CCR5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse CCR5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) CCR1, rmCCR4, and rmCCR8 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse CCR5 by Western Blot. Western blot shows lysates of EL-4 mouse lymphoblast cell line (IL-2 secreting) and mouse thymus tissue. PVDF Membrane was probed with 1 µg/mL of Mouse CCR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6138) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR5 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
