Explore
Validate
Learn12-1956-41
antibody from Invitrogen Antibodies
Targeting: CCR5
CC-CKR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 12-1956-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD195 (CCR5) Monoclonal Antibody (NP-6G4), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The NP-6G4 monoclonal antibody reacts with human CD195 (CCR5), an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1a, MIP-1b and RANTES. Human CCR5 is expressed on resting T cells with a memory/effector phenotype, monocytes, macrophages and immature dendritic cells. CCR5 functions as a receptor to direct the chemotaxis of lymphocytes, and is the primary co-receptor for macrophage-tropic Human Immunodeficiency Virus Type 1 (HIV-1), which binds CCR5 through gp120. Applications Reported: This NP-6G4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This NP-6G4 antibody has been pre-titrated and tested on normal human peripheral blood cells. For optimal performance, it is recommended that staining with NP-6G4 be done at 37°C for 1 hour in Flow Cytometry Staining Buffer (cat. 00-4222). This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- NP-6G4
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.
Failure to upregulate cell surface PD-1 is associated with dysregulated stimulation of T cells by TGN1412-like CD28 superagonist.
Sommer A, Fabri M
PloS one 2015;10(6):e0130395
PloS one 2015;10(6):e0130395
Failure to upregulate cell surface PD-1 is associated with dysregulated stimulation of T cells by TGN1412-like CD28 superagonist.
Thaventhiran T, Alhumeed N, Yeang HX, Sethu S, Downey JS, Alghanem AF, Olayanju A, Smith EL, Cross MJ, Webb SD, Williams DP, Bristow A, Ball C, Stebbings R, Sathish JG
mAbs 2014;6(5):1290-9
mAbs 2014;6(5):1290-9
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0037-42) and 0.125 µg of Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or 0.125 µg of Anti-Human CD195 (CCR5) PE (right). Staining was performed at 37°C for hour and cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye
