Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-38300 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-MYPT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MYPT1 in extracts from NIH-3T3 cells using a MYPT1 polyclonal antibody (Product # PA5-38300).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of MYPT1 was achieved by transfecting NTERA-2.cl.D1 cells with MYPT1 specific siRNA (Silencer® select Product # s9237). Western blot analysis (Fig. a) was performed using whole cell extracts from the MYPT1 knockdown cells (lane 2) and non-specific scrambled siRNA transfected cells (lane 1). The blot was probed with c-Cbl Polyclonal Antibody (Product # PA5-38300, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MYPT1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MYPT1 polyclonal Antibody (Product # PA5-38300) and a 130 kDa band corresponding to MYPT1 was observed in Mouse Ovary, HEK-293T and NTERA-2.cl.D1. Tissue lysate (30 µg lysate) of Mouse Ovary (Lane 1), whole cell extracts (30 µg lysate) of HEK-293T (Lane 2) and NTERA-2.cl.D1 (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MYPT1 in paraffin-embedded human brain tissue using a MYPT1 polyclonal antibody (Product # PA5-38300).