AM03116PU-S

antibody from OriGene

Targeting: NT5E CALJA, CD73, eN, eNT, NT5

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

AM03116PU-S

Provider

OriGene

Product name

CD73 (NT5E) (27-252) mouse monoclonal antibody, clone AT89E9, Purified

Antibody type

Monoclonal

Description

CD73 (NT5E) (27-252) mouse monoclonal antibody, clone AT89E9, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

NT5E

Isotype

IgG

Antibody clone number

AT89E9

Vial size

50 µl

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis: Cell lysates of human mesenchymal stem cell (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CD73 antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry: -N CD73 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Pancreas at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry: -N CD73 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Adrenal at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Validation comment

IHC