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Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [17]
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- Validations
- Flow cytometry [4]
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- Product number
- NBP2-48480 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal 5'-Nucleotidase/CD73 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells.
Characterization of Gaucher disease bone marrow mesenchymal stromal cells reveals an altered inflammatory secretome.
CD38 stimulation lowers the activation threshold and enhances the alloreactivity of cord blood T cells by activating the phosphatidylinositol 3-kinase pathway and inducing CD73 expression.
Insights into thymic purine metabolism and adenosine deaminase deficiency revealed by transgenic mice overexpressing ecto-5'-nucleotidase (CD73).
Characterization of human thymic epithelial cell surface antigens: phenotypic similarity of thymic epithelial cells to epidermal keratinocytes.
Ecto-5'-nucleotidase activity is not required for T cell activation through CD73.
Characterization of a cell surface glycoprotein IPO-3, expressed on activated human B and T lymphocytes.
Characterization of an IgM Fc-binding receptor on human T cells.
Phenotype and topography of human thymic B cells. An immunohistologic study.
5'-nucleotidase--an overview of the last three years.
Expression of ecto-5'-nucleotidase (CD73) in normal mammary gland and in breast carcinoma.
Human B cells: differentiation and neoplasia.
T-cell antigen CD28 mediates adhesion with B cells by interacting with activation antigen B7/BB-1.
T-cell antigen CD28 mediates adhesion with B cells by interacting with activation antigen B7/BB-1.
Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73).
Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73).
Report from Vienna: in search of all surface molecules expressed on human leukocytes.
Samanta D, Park Y, Ni X, Li H, Zahnow CA, Gabrielson E, Pan F, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2018 Feb 6;115(6):E1239-E1248
Proceedings of the National Academy of Sciences of the United States of America 2018 Feb 6;115(6):E1239-E1248
Characterization of Gaucher disease bone marrow mesenchymal stromal cells reveals an altered inflammatory secretome.
Campeau PM, Rafei M, Boivin MN, Sun Y, Grabowski GA, Galipeau J
Blood 2009 Oct 8;114(15):3181-90
Blood 2009 Oct 8;114(15):3181-90
CD38 stimulation lowers the activation threshold and enhances the alloreactivity of cord blood T cells by activating the phosphatidylinositol 3-kinase pathway and inducing CD73 expression.
Borrione P, Peola S, Mariani S, Besostri B, Mallone R, Malavasi F, Pileri A, Massaia M
Journal of immunology (Baltimore, Md. : 1950) 1999 May 15;162(10):6238-46
Journal of immunology (Baltimore, Md. : 1950) 1999 May 15;162(10):6238-46
Insights into thymic purine metabolism and adenosine deaminase deficiency revealed by transgenic mice overexpressing ecto-5'-nucleotidase (CD73).
Resta R, Hooker SW, Laurent AB, Jamshedur Rahman SM, Franklin M, Knudsen TB, Nadon NL, Thompson LF
The Journal of clinical investigation 1997 Feb 15;99(4):676-83
The Journal of clinical investigation 1997 Feb 15;99(4):676-83
Characterization of human thymic epithelial cell surface antigens: phenotypic similarity of thymic epithelial cells to epidermal keratinocytes.
Patel DD, Whichard LP, Radcliff G, Denning SM, Haynes BF
Journal of clinical immunology 1995 Mar;15(2):80-92
Journal of clinical immunology 1995 Mar;15(2):80-92
Ecto-5'-nucleotidase activity is not required for T cell activation through CD73.
Gutensohn W, Resta R, Misumi Y, Ikehara Y, Thompson LF
Cellular immunology 1995 Apr 1;161(2):213-7
Cellular immunology 1995 Apr 1;161(2):213-7
Characterization of a cell surface glycoprotein IPO-3, expressed on activated human B and T lymphocytes.
Sidorenko SP, Clark EA
Journal of immunology (Baltimore, Md. : 1950) 1993 Nov 1;151(9):4614-24
Journal of immunology (Baltimore, Md. : 1950) 1993 Nov 1;151(9):4614-24
Characterization of an IgM Fc-binding receptor on human T cells.
Nakamura T, Kubagawa H, Ohno T, Cooper MD
Journal of immunology (Baltimore, Md. : 1950) 1993 Dec 15;151(12):6933-41
Journal of immunology (Baltimore, Md. : 1950) 1993 Dec 15;151(12):6933-41
Phenotype and topography of human thymic B cells. An immunohistologic study.
Fend F, Nachbaur D, Oberwasserlechner F, Kreczy A, Huber H, Müller-Hermelink HK
Virchows Archiv. B, Cell pathology including molecular pathology 1991;60(6):381-8
Virchows Archiv. B, Cell pathology including molecular pathology 1991;60(6):381-8
5'-nucleotidase--an overview of the last three years.
Thompson LF
Advances in experimental medicine and biology 1991;309B:145-50
Advances in experimental medicine and biology 1991;309B:145-50
Expression of ecto-5'-nucleotidase (CD73) in normal mammary gland and in breast carcinoma.
Krüger KH, Thompson LF, Kaufmann M, Möller P
British journal of cancer 1991 Jan;63(1):114-8
British journal of cancer 1991 Jan;63(1):114-8
Human B cells: differentiation and neoplasia.
Mulligan SP
Leukemia & lymphoma 1990;1(5-6):275-91
Leukemia & lymphoma 1990;1(5-6):275-91
T-cell antigen CD28 mediates adhesion with B cells by interacting with activation antigen B7/BB-1.
Linsley PS, Clark EA, Ledbetter JA
Proceedings of the National Academy of Sciences of the United States of America 1990 Jul;87(13):5031-5
Proceedings of the National Academy of Sciences of the United States of America 1990 Jul;87(13):5031-5
T-cell antigen CD28 mediates adhesion with B cells by interacting with activation antigen B7/BB-1.
Linsley PS, Clark EA, Ledbetter JA
Proceedings of the National Academy of Sciences of the United States of America 1990 Jul;87(13):5031-5
Proceedings of the National Academy of Sciences of the United States of America 1990 Jul;87(13):5031-5
Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73).
Thomson LF, Ruedi JM, Glass A, Moldenhauer G, Moller P, Low MG, Klemens MR, Massaia M, Lucas AH
Tissue antigens 1990 Jan;35(1):9-19
Tissue antigens 1990 Jan;35(1):9-19
Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73).
Thomson LF, Ruedi JM, Glass A, Moldenhauer G, Moller P, Low MG, Klemens MR, Massaia M, Lucas AH
Tissue antigens 1990 Jan;35(1):9-19
Tissue antigens 1990 Jan;35(1):9-19
Report from Vienna: in search of all surface molecules expressed on human leukocytes.
Clark EA, Lanier LL
Journal of clinical immunology 1989 Jul;9(4):265-72
Journal of clinical immunology 1989 Jul;9(4):265-72
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A cell surface stain was performed on U87MG cells with NBP2-48480APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on U87 MG cells with 5'-Nucleotidase/CD73 Antibody (AD2) antibody NBP2-48480 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on CD3+ human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25186PCP.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647.
