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- References [8]
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- Western blot [1]
- Immunohistochemistry [6]
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- Product number
- NBP1-85740 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85740, RRID:AB_11030662
- Product name
- Rabbit Polyclonal 5'-Nucleotidase/CD73 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human 5'-Nucleotidase/CD73 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references HPV-16 Infection Is Associated with a High Content of CD39 and CD73 Ectonucleotidases in Cervical Samples from Patients with CIN-1.
Human adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse.
Human ectonucleotidase-expressing CD25(high) Th17 cells accumulate in breast cancer tumors and exert immunosuppressive functions.
Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1.
Potentiation of temozolomide antitumor effect by purine receptor ligands able to restrain the in vitro growth of human glioblastoma stem cells.
Loss of tenomodulin results in reduced self-renewal and augmented senescence of tendon stem/progenitor cells.
Alternative splicing of human NT5E in cirrhosis and hepatocellular carcinoma produces a negative regulator of ecto-5'-nucleotidase (CD73).
CD73-generated extracellular adenosine in chronic lymphocytic leukemia creates local conditions counteracting drug-induced cell death.
de Lourdes Mora-García M, López-Cisneros S, Gutiérrez-Serrano V, García-Rocha R, Weiss-Steider B, Hernández-Montes J, Sánchez-Peña HI, Ávila-Ibarra LR, Don-López CA, Muñóz-Godínez R, Pineda DBT, Chacón-Salinas R, Vallejo-Castillo L, Pérez-Tapia SM, Monroy-García A
Mediators of inflammation 2019;2019:4651627
Mediators of inflammation 2019;2019:4651627
Human adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse.
Calabrese G, Giuffrida R, Forte S, Fabbi C, Figallo E, Salvatorelli L, Memeo L, Parenti R, Gulisano M, Gulino R
Scientific reports 2017 Aug 2;7(1):7110
Scientific reports 2017 Aug 2;7(1):7110
Human ectonucleotidase-expressing CD25(high) Th17 cells accumulate in breast cancer tumors and exert immunosuppressive functions.
Thibaudin M, Chaix M, Boidot R, Végran F, Derangère V, Limagne E, Berger H, Ladoire S, Apetoh L, Ghiringhelli F
Oncoimmunology 2016;5(1):e1055444
Oncoimmunology 2016;5(1):e1055444
Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1.
Bullen JW, Tchernyshyov I, Holewinski RJ, DeVine L, Wu F, Venkatraman V, Kass DL, Cole RN, Van Eyk J, Semenza GL
Science signaling 2016 May 31;9(430):ra56
Science signaling 2016 May 31;9(430):ra56
Potentiation of temozolomide antitumor effect by purine receptor ligands able to restrain the in vitro growth of human glioblastoma stem cells.
D'Alimonte I, Nargi E, Zuccarini M, Lanuti P, Di Iorio P, Giuliani P, Ricci-Vitiani L, Pallini R, Caciagli F, Ciccarelli R
Purinergic signalling 2015 Sep;11(3):331-46
Purinergic signalling 2015 Sep;11(3):331-46
Loss of tenomodulin results in reduced self-renewal and augmented senescence of tendon stem/progenitor cells.
Alberton P, Dex S, Popov C, Shukunami C, Schieker M, Docheva D
Stem cells and development 2015 Mar 1;24(5):597-609
Stem cells and development 2015 Mar 1;24(5):597-609
Alternative splicing of human NT5E in cirrhosis and hepatocellular carcinoma produces a negative regulator of ecto-5'-nucleotidase (CD73).
Snider NT, Altshuler PJ, Wan S, Welling TH, Cavalcoli J, Omary MB
Molecular biology of the cell 2014 Dec 15;25(25):4024-33
Molecular biology of the cell 2014 Dec 15;25(25):4024-33
CD73-generated extracellular adenosine in chronic lymphocytic leukemia creates local conditions counteracting drug-induced cell death.
Serra S, Horenstein AL, Vaisitti T, Brusa D, Rossi D, Laurenti L, D'Arena G, Coscia M, Tripodo C, Inghirami G, Robson SC, Gaidano G, Malavasi F, Deaglio S
Blood 2011 Dec 1;118(23):6141-52
Blood 2011 Dec 1;118(23):6141-52
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Analysis in human cell line U-87 MG.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human aortic valve staining for CD73. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining for pig aortic valve. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human endometrium shows strong membranous positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human duodenum shows strong positivity in apical membrane in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human endometrium shows strong positivity in apical membrane in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human liver shows strong membranous positivity in hepatocytes.
