PA5-29750

antibody from Invitrogen Antibodies

Targeting: NT5E CALJA, CD73, eN, eNT, NT5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29750

Provider

Invitrogen Antibodies

Product name

CD73 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2, HCT116, Mouse placenta.

Concentration

0.28 mg/mL

References

Submitted references

Mesenchymal stem/stromal cells-derived IL-6 promotes nasopharyngeal carcinoma growth and resistance to cisplatin via upregulating CD73 expression.

Zeng J, Chen S, Li C, Ye Z, Lin B, Liang Y, Wang B, Ma Y, Chai X, Zhang X, Zhou K, Zhang Q, Zhang H
Journal of Cancer 2020;11(8):2068-2079

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD73 in Mouse tissue extracts (50 µg). Samples was separated by 12% SDS-PAGE and the membrane was probed with CD73 Polyclonal antibody (Product # PA5-29750) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CD73 Polyclonal Antibody (Product # PA5-29750). Sample (30 µg of whole cell lysate). Lane A: HepG2. Lane B: HCT116. 12% SDS PAGE. CD73 Polyclonal Antibody (Product # PA5-29750) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CD73 Polyclonal Antibody (Product # PA5-29750). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 Polyclonal Antibody (Product # PA5-29750) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD73 Polyclonal Antibody detects CD73 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD73 Polyclonal Antibody (Product # PA5-29750) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD73 Polyclonal Antibody detects CD73 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Green: CD73 stained by CD73 Polyclonal Antibody (Product # PA5-29750) diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD73 (Product # PA5-29750) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.