ABIN457937

antibody from antibodies-online

Targeting: ATP11C ATPIG, ATPIQ

No application information provided.

Antibody Data

Product number

ABIN457937

Provider

antibodies-online

Proper citation

Antibodies-Online Cat#ABIN457937, RRID:AB_10780803

Product name

anti-Ig (Joining Chain) antibody

Antibody type

Polyclonal

Antigen

Human J chain is a polypeptide folded within the polymeric structure of the immunoglobulin. J chain isolated form human polymeric IgA and IgM are identical by criteria of composition, peptide maps and antigenicity. Human J chain has been established as distinct from all other component chains of polymeric IgA and IgM. It has a unique primary structure. Antisera to light chains or heavy chains of human Immunoglobulins do not react with J chain. J chain is released from dimeric human myeloma IgA by sulphitolysis and purified by ion exchange chromatography in urea and gel filtration in guanidine. Its purity is tested in alkaline urea polyacrylamide gel electrophoresis, in immunodiffusion and immunoelectrophoresis, showing it to be free of IgA, heavy or light chain contaminants. Freund€™s complete adjuvant is used in the first step of the immunization procedure.

Reactivity

Human

Host

Goat

Vial size

0.5 ml

Concentration

Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested in agar-block immunodiffusion titration against a 1% solution of purifi

Storage

Lyophilised at +4°C (10 years), reconstituted at or below -20°C (3-5 years), reconstituted at +4°C at least 7 days