AF6568

antibody from Novus Biologicals

Targeting: BLVRB FLR, SDR43U1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6568

Provider

Novus Biologicals

Product name

Sheep Polyclonal Biliverdin Reductase B/BLVRB Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human, mouse, and rat Biliverdin Reductase B/BLVRB in Western blots.

Reactivity

Human, Mouse, Rat

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

In silico and crystallographic studies identify key structural features of biliverdin IXβ reductase inhibitors having nanomolar potency.

Nesbitt NM, Zheng X, Li Z, Manso JA, Yen WY, Malone LE, Ripoll-Rozada J, Pereira PJB, Mantle TJ, Wang J, Bahou WF
The Journal of biological chemistry 2018 Apr 13;293(15):5431-5446

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human, Mouse, and Rat Biliverdin Reductase B/BLVRB by Western Blot. Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Biliverdin Reductase B/BLVRB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6568) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Biliverdin Reductase B/BLVRB at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.