ABIN2508220

antibody from antibodies-online

Targeting: GYPA CD235a, GPA, MN, MNS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN2508220

Provider

antibodies-online

Product name

anti-Glycophorin A (MNS Blood Group) (GYPA) antibody

Antibody type

Monoclonal

Antigen

Human erythrocytes treated with neuraminidase

Reactivity

Human

Host

Mouse

Isotype

IgM

Antibody clone number

GPHN02

Vial size

100 μg

Storage

Store at 2 to 8°C.Antibody is stable for 24 months.

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Image(s): Western Blotting

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (p/n 611-1102). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (p/n 611-1102) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. Western Blot of Goat anti-Rabbit IgG Rhodamine Conjugated Secondary Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None. Dot Blot of Rhodamine Conjugated Goat-anti-Rabbit IgG. Antigen: Rabbit IgG. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng. Primary antibody: none. Secondary antibody: Rhodamine Conjugated Goat-a-Rabbit IgG secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (p/n 611-1102). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (p/n 611-1102) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. Western Blot of Goat anti-Rabbit IgG Rhodamine Conjugated Secondary Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None. Dot Blot of Rhodamine Conjugated Goat-anti-Rabbit IgG. Antigen: Rabbit IgG. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng. Primary antibody: none. Secondary antibody: Rhodamine Conjugated Goat-a-Rabbit IgG secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.