11-9886-42

antibody from Invitrogen Antibodies

Targeting: GYPA CD235a, GPA, MN, MNS

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11-9886-42

Provider

Invitrogen Antibodies

Product name

CD235a (Glycophorin A) Monoclonal Antibody (10F7MN), FITC, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The monoclonal antibody 10F7MN recognizes human glycophorin A (also known as CD235a). The antibody can see both the M and N alleles. Glycophorin A is a 151 amino acid sialoglycoprotein found on the erythrocyte (RBC) and erthyroid progenitor cell membrane at about 500,000 copies per cell. The gene for glycophorin resides on chromosome 4 and has 2 allelic forms: M and N, which differ in two amino acids. The M group possesses Ser1 and Gly5 while the N group has Leu1 and Glu5. Recent data suggest that exposure to toxins can cause mutation or loss of an allele resulting in phenotypic changes. Studies are also beginning to correlate genotype/phenotype with predisposition to cancer and heart disease.

Conjugate

Green dye

Antibody clone number

10F7MN

Concentration

5 µL/Test

References

Submitted references

Antitumor Effect and Immune Response of Nanosecond Pulsed Electric Fields in Pancreatic Cancer.

Zhao J, Chen S, Zhu L, Zhang L, Liu J, Xu D, Tian G, Jiang T
Frontiers in oncology 2020;10:621092

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Erythrocyte sialoglycoproteins engage Siglec-9 on neutrophils to suppress activation.

Lizcano A, Secundino I, Döhrmann S, Corriden R, Rohena C, Diaz S, Ghosh P, Deng L, Nizet V, Varki A
Blood 2017 Jun 8;129(23):3100-3110

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Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients.

Beck Z, Jagodzinski LL, Eller MA, Thelian D, Matyas GR, Kunz AN, Alving CR
PloS one 2013;8(11):e81002

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Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow.

Lansdorp PM, Dragowska W
The Journal of experimental medicine 1992 Jun 1;175(6):1501-9

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Flow cytometric characterization of normal and variant cells with monoclonal antibodies specific for glycophorin A.

Langlois RG, Bigbee WL, Jensen RH
Journal of immunology (Baltimore, Md. : 1950) 1985 Jun;134(6):4009-17

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of normal human peripheral blood cells with Anti-Human CD235 eFluor® 450 and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human Glycophorin A (MN) FITC (right). Red blood cell gate was used for analysis.

Conjugate

Green dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Green dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Profiling of immune suppressive cells after nsPEFs treatment. (A) Frequency of Treg cells in response to nsPEFs treatment in the spleen on day 3 and 7. Analyses were performed on CD4 + cells. (B) Frequency of MDSCs (CD11b + Ly6G + and CD11b + Ly6C + ) and macrophages (CD11b + F4/80 + ) in the tumor on day 3 and 7. (C) Frequency of MDSC (CD11b + Ly6G + and CD11b + Ly6C + ) and macrophages (CD11b + F4/80 + ) in the spleen on day 3 and 7. (D) Frequency of MDSC (CD11b + Ly6G + and CD11b + Ly6C + ) and macrophages (CD11b + F4/80 + ) in the tumor on day 3 and 7. Data are presented as the mean +- SD. P-values were calculated based on a Student's t -test (n = 4 per group). * p < 0.05, ** p < 0.01.

Conjugate

Green dye