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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB5235 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal Semaphorin 4D/CD100 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Semaphorin 4D in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Semaphorin 3F is observed.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Activation of stimulator of interferon genes (STING) induces ADAM17-mediated shedding of the immune semaphorin SEMA4D.
Motani K, Kosako H
The Journal of biological chemistry 2018 May 18;293(20):7717-7726
The Journal of biological chemistry 2018 May 18;293(20):7717-7726
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Mouse Semaphorin 4D/CD100 by Western Blot. Western blot shows lysates of mouse brain and thymus tissue under reducing (R) and non-reducing (NR) conditions. PVDF membrane was probed with 2 µg/mL Rat Anti-Mouse Semaphorin 4D/CD100 Monoclonal Antibody (Catalog # MAB5235) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for Semaphorin 4D/CD100 was detected at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
