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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [3]
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Validation data
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- Product number
- PA5-46904 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD268 (BAFF Receptor) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse BAFF R and less than 2% cross-reactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhNGF R, rhOPG, rhRANK, rhTNF RI, and rhTNF RII is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD268 (BAFF Receptor) in immersion fixed paraffin-embedded sections of human spleen. Samples were incubated with CD268 (BAFF Receptor) polyclonal antibody (Product # PA5-46904) using a dilution of 3 µg/mL for 1 hour at room temperature followed by Anti-Goat IgG VisUCyte™ HRP Polymer Antibody. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic . Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CD268 (BAFF Receptor) in Peripheral blood mononuclear cells (PBMCs). Samples were incubated in either (A) CD268 (BAFF Receptor) polyclonal antibody (Product # PA5-46904) or (B) Normal Goat IgG Control followed by APC-conjugated anti-goat secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of human BAFF Receptor by is shown by increase in RFU (relative fluorescent units - measure of inhibition of BAFF activity) with increasing concentrations of CD268 (BAFF Receptor) polyclonal antibody (PA5-46904).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of CD268 (BAFF Receptor) in mouse B cells. Samples were incubated in CD268 (BAFF Receptor) polyclonal antibody (Product # PA5-46904). Recombinant Human BAFF R/TNFRSF13C Fc Chimera inhibits Recombinant Human BAFF/BLyS/TNFSF13B induced proliferation in mouse B cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human BAFF/BLyS/TNFSF13B (5 ng/mL) activity elicited by Recombinant Human BAFF R/TNFRSF13C Fc Chimera (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BAFF R/TNFRSF13C Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.1-1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of CD268 (BAFF Receptor) in mouse B cells. Samples were incubated in CD268 (BAFF Receptor) polyclonal antibody (Product # PA5-46904). Recombinant Human BAFF R/TNFRSF13C Fc Chimera inhibits Recombinant Human BAFF/BLyS/TNFSF13B induced proliferation in mouse B cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human BAFF/BLyS/TNFSF13B (5 ng/mL) activity elicited by Recombinant Human BAFF R/TNFRSF13C Fc Chimera (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti‚Human BAFF R/TNFRSF13C Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 3-15 µg/mL.
