LS-C150029

antibody from LSBio

Targeting: AXL ARK, JTK11, Tyro7, UFO

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C150029

Provider

LSBio

Product name

Anti-AXL Antibody (phospho-Tyr779) LS-C150029

Antibody type

Polyclonal

Description

Protein A purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

25µg

Storage

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Phospho-Axl (Y779) in A172 Human Cell Line. Axl phosphorylated at Y779 (panels B, D) and total Axl (panels A, C) were assessed in immersion fixed A172 human glioblastoma cells incubated with (panels C, D ) or without ( panels A, B) pervanadate. PhosphoAxl was detected using Rabbit Anti-Human PhosphoAxl (Y779) Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red, panels B, D) and counterstained using DAPI (blue). Total Axl was detected using Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody. Cells were stained using NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green, panels A, C). Specific staining was localized to cytoplasm.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Phospho-Axl (Y779) in Human Stomach Cancer Tissue. Axl phosphorylated at site Y779 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Human Phospho-Axl (Y779) Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Tissue was stained using Anti-Rabbit HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells.