PA5-30479

antibody from Invitrogen Antibodies

Targeting: CORO1C coronin-3, HCRNN4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30479

Provider

Invitrogen Antibodies

Product name

Coronin 3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A549, HeLa, HepG2, HCT116, NIH-3T3, mouse brain.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Coronin 3 Polyclonal Antibody (Product # PA5-30479). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 10% SDS PAGE. Coronin 3 Polyclonal Antibody (Product # PA5-30479) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Coronin 3 Polyclonal Antibody (Product # PA5-30479). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. Coronin 3 Polyclonal Antibody (Product # PA5-30479) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Coronin 3 Polyclonal Antibody (Product # PA5-30479). Sample (30 µg of whole cell lysate). Lane A: HeLa. 10% SDS PAGE. Coronin 3 Polyclonal Antibody (Product # PA5-30479) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Coronin 1C in methanol-fixed HeLa cells using a Coronin 1C polyclonal antibody (Product # PA5-30479) at a 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Coronin 3 Polyclonal Antibody detects Coronin 1C protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Coronin 3 Polyclonal Antibody (Product # PA5-30479) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.