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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Western blot [2]
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- Product number
- PA1-4661 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLRA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is specific for the ~48 kDa alpha 1 and alpha 2 subunits of the Glycine Receptor in Western blots of rat spinal cord and brain stem and in cell extracts, and does not recognize other Glycine Receptor subunits. Immunolabeling is blocked by preadsorption of antibody with the peptide immunogen.
Submitted references Role of an Absolutely Conserved Tryptophan Pair in the Extracellular Domain of Cys-Loop Receptors.
Braun N, Lynagh T, Yu R, Biggin PC, Pless SA
ACS chemical neuroscience 2016 Mar 16;7(3):339-48
ACS chemical neuroscience 2016 Mar 16;7(3):339-48
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of 10 µg of rat spinal cord. As shown in the autoradiograph, the Anti-Glycine Receptor (Product # PA1-4661) is specific for the ~48 kDa subunits recognizing both the alpha1- and alpha2-subunits of the Glycine Receptor.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of GLRA1 in rat spinal cord lysate showing specific immunolabeling of a band at ~48 kDa corresponding to the alpha 1 and alpha 2-subunits of GLRA1 polyclonal antibody (Product # PA1-4661). Immunolabeling was absent from a rat hippocampal lysate (H), as the glycine receptor is not expressed in the hippocampus.
