PA5-29811

antibody from Invitrogen Antibodies

Targeting: GSTA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29811

Provider

Invitrogen Antibodies

Product name

GSTA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2, mouse liver.

Concentration

0.86 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GSTA1 using A. 10 µg mouse liver lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a GSTA1 polyclonal antibody (Product # PA5-29811) at a dilution of 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GSTA1 using 30 µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a GSTA1 polyclonal antibody (Product # PA5-29811) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GSTA1 was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a GSTA1 Polyclonal Antibody (Product # PA5-29811) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GSTA1 in methanol-fixed Hep3B cells using a GSTA1 polyclonal antibody (Product # PA5-29811) at a 1:500 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

GSTA1 Polyclonal Antibody detects GSTA1 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSTA1 stained by GSTA1 Polyclonal Antibody (Product # PA5-29811) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded PC3 xenograft, using GSTA1 (Product # PA5-29811) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.