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Learn35-5300
antibody from Invitrogen Antibodies
Targeting: MAPT
DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, PPP1R103, tau
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [5]
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- Product number
- 35-5300 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Tau (Ser396) Monoclonal Antibody (PHF13.6)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This monoclonal antibody recognizes phosphorylation-dependent epitopes of ser-396 on PHF-tau as well as fetal tau, but does not recognize autopsy-derived normal adult tau. Phosphoamino ser-396 is one of the two major immunodominant residues of PHF-tau. PHF 13.6 does not recognize unphosphorylated peptide 390-408 or the same peptide phosphorylated at ser-400, thr-403, or ser-404. PHF 13.6 specifically recognizes the ser-396 phosphorylated peptide and all diphosphorylated peptides containing phosphorylated ser-396. Reactivity was confirmed with human PHF-Tau.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- PHF13.6
- Vial size
- 50 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references Repetitive Model of Mild Traumatic Brain Injury Produces Cortical Abnormalities Detectable by Magnetic Resonance Diffusion Imaging, Histopathology, and Behavior.
Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment.
HS3ST2 expression is critical for the abnormal phosphorylation of tau in Alzheimer's disease-related tau pathology.
Lovastatin suppresses the aberrant tau phosphorylation from FTDP-17 mutation and okadaic acid-induction in rat primary neurons.
Effects of antibodies to phosphorylated and non-phosphorylated tau on in vitro tau phosphorylation at Serine-199: Preliminary report.
Deficient hippocampal insulin signaling and augmented Tau phosphorylation is related to obesity- and age-induced peripheral insulin resistance: a study in Zucker rats.
Transgenic mouse and cell culture models demonstrate a lack of mechanistic connection between endoplasmic reticulum stress and tau dysfunction.
Ubiquitin-positive neuronal and tau 2-positive glial inclusions in frontotemporal dementia of motor neuron type.
Unique Alzheimer's disease paired helical filament specific epitopes involve double phosphorylation at specific sites.
Yu F, Shukla DK, Armstrong RC, Marion CM, Radomski KL, Selwyn RG, Dardzinski BJ
Journal of neurotrauma 2017 Apr 1;34(7):1364-1381
Journal of neurotrauma 2017 Apr 1;34(7):1364-1381
Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment.
Smolek T, Madari A, Farbakova J, Kandrac O, Jadhav S, Cente M, Brezovakova V, Novak M, Zilka N
The Journal of comparative neurology 2016 Mar 1;524(4):874-95
The Journal of comparative neurology 2016 Mar 1;524(4):874-95
HS3ST2 expression is critical for the abnormal phosphorylation of tau in Alzheimer's disease-related tau pathology.
Sepulveda-Diaz JE, Alavi Naini SM, Huynh MB, Ouidja MO, Yanicostas C, Chantepie S, Villares J, Lamari F, Jospin E, van Kuppevelt TH, Mensah-Nyagan AG, Raisman-Vozari R, Soussi-Yanicostas N, Papy-Garcia D
Brain : a journal of neurology 2015 May;138(Pt 5):1339-54
Brain : a journal of neurology 2015 May;138(Pt 5):1339-54
Lovastatin suppresses the aberrant tau phosphorylation from FTDP-17 mutation and okadaic acid-induction in rat primary neurons.
Li R, Xu DE, Ma T
Neuroscience 2015 May 21;294:14-20
Neuroscience 2015 May 21;294:14-20
Effects of antibodies to phosphorylated and non-phosphorylated tau on in vitro tau phosphorylation at Serine-199: Preliminary report.
Loeffler DA, Smith LM, Klaver AC, Martić S
Experimental gerontology 2015 Jul;67:15-8
Experimental gerontology 2015 Jul;67:15-8
Deficient hippocampal insulin signaling and augmented Tau phosphorylation is related to obesity- and age-induced peripheral insulin resistance: a study in Zucker rats.
Špolcová A, Mikulášková B, Kršková K, Gajdošechová L, Zórad Š, Olszanecki R, Suski M, Bujak-Giżycka B, Železná B, Maletínská L
BMC neuroscience 2014 Sep 25;15:111
BMC neuroscience 2014 Sep 25;15:111
Transgenic mouse and cell culture models demonstrate a lack of mechanistic connection between endoplasmic reticulum stress and tau dysfunction.
Spatara ML, Robinson AS
Journal of neuroscience research 2010 Jul;88(9):1951-61
Journal of neuroscience research 2010 Jul;88(9):1951-61
Ubiquitin-positive neuronal and tau 2-positive glial inclusions in frontotemporal dementia of motor neuron type.
Forno LS, Langston JW, Herrick MK, Wilson JD, Murayama S
Acta neuropathologica 2002 Jun;103(6):599-606
Acta neuropathologica 2002 Jun;103(6):599-606
Unique Alzheimer's disease paired helical filament specific epitopes involve double phosphorylation at specific sites.
Hoffmann R, Lee VM, Leight S, Varga I, Otvos L Jr
Biochemistry 1997 Jul 1;36(26):8114-24
Biochemistry 1997 Jul 1;36(26):8114-24
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Phospho-Tau (Ser396) Monoclonal Antibody (PHF13.6) (Product # 35-5300) and a 50kDa band corresponding to Phospho-Tau (Ser396) was observed across cell line tested and increased upon Retinoic acid and the successive Okadaic acid treatments. Whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), SH-SY5Y treated with Retinoic acid (10 uM for 8 days) (Lane 2), SH-SY5Y differentiated with Retinoic acid and treated with Okadoic acid (100 nM for 6 hours) (Lane 3) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:5000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Hyperphosphorylation of Tau protein on different epitopes in hippocampi of fa/fa rats and their control. A Western blots of phosphorylation of Tau protein on the epitopes Ser396 and Thr231 (n = 6 rats per group) B Densitometric quantification of western blots normalized to beta-actin: data are mean +- SD (n = 6 rats per group). Statistical analysis was calculated by two-way ANOVA, Bonferroni post hoc test.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIG. 7. Tau pathology is not observed after r-mTBI. Immunohistochemistry for phosphorylated serine 396 tau shows a lack of immunolabeling in the cortex of mice perfused post-imaging at 6 weeks after r-mTBI ( A ). In contrast, the positive control P301S human tau tissues show high levels of phosphorylated serine 396 tau in cortical neurons ( B ). Scale bar = 100 mum. r-mTBI, repetitive mild traumatic brain injury.
