NBP2-50039
antibody from Novus Biologicals
Targeting: AURKB
Aik2, AIM-1, ARK2, AurB, IPL1, PPP1R48, STK12, STK5
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NBP2-50039 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Aurora B Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This antibody does not cross-react with Aurora A or Aurora C.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aurora B Antibody (3F11) [NBP2-50039] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with NBP2-50039. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. NBP2-50039 binds strongly to Aurora B at 38 kDa. Right: Blot of recombinant human Aurora A, B and C were probed with NBP2-50039.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aurora B Antibody (3F11) [NBP2-50039] - Analysis of different cell lysates and recombinant protein solutions using mouse mAb to Aurora B. Left: cells were treated with 100ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] mouse KR158 cells. Right: Blot of purified full length recombinant human aurora A, B and C were probed with NBP2-50039. The antibody binds specifically only to aurora B and not to the closely related aurora A and C.