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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- ABIN1580427 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-High-Mobility Group Box 1 (HMGB1) antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- affinity purified antibody
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1F3
- Vial size
- 100 μL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term or -20°C long term.
- Handling
- Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Blot of crude HeLa cell extract stained with MCS-1F3. HMGB1 runs at an apparent molecular weight of 25kDa.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- HeLa cells grown in tissue culture and stained with ABIN1580427 (red), chicken polyclonal antibody to Vimentin CPCA-VIM (green) and DNA (blue). The ABIN1580427 antibody reveals strong nuclear staining which overlaps with the DNA stain.
