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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AM33095PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti HMGB1 (C-term)
- Antibody type
- Monoclonal
- Antigen
- Recombinant Human HMGB1 with GST tag. A universal T cell epitope from a Mycobacterium tuberculosis antigen was introduced into the C-terminus of HMGB1 to increase the immunogenicity.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3E8
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Hela cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-HMGB1 Antibody Cat.-No AM33095PU (Clone 3E8). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Hela cells were stained with anti-HMGB1 Antibody Cat.-No AM33095PU (Clone 3E8), followed by DyLight⢠549 conjugated anti-mouse IgG. Nuclei were stained with DAPI.
