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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AM09063PU-N - Provider product page
- Provider
- OriGene
- Product name
- HMG1 (HMGB1) mouse monoclonal antibody, clone J2E1, Purified
- Antibody type
- Monoclonal
- Description
- HMG1 (HMGB1) mouse monoclonal antibody, clone J2E1, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HMGB1
- Isotype
- IgG
- Antibody clone number
- J2E1
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis: Cell lysates of Jurkat and HeLa (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human HMGB1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: Paraffin embedded sections of human colon cancer tissue were incubated with anti-human HMGB1 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate bufferand detected using Diaminobenzidine (DAB).
- Validation comment
- IHC
