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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00003146-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003146-M03, RRID:AB_539584
- Product name
- Mouse Monoclonal HMGB1/HMG-1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. HMGB1 - high-mobility group box 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Anti-inflammatory effects of hyperoside in human endothelial cells and in mice.
Proteomic analysis of human epithelial lining fluid by microfluidics-based nanoLC-MS/MS: a feasibility study.
Ku SK, Zhou W, Lee W, Han MS, Na M, Bae JS
Inflammation 2015 Apr;38(2):784-99
Inflammation 2015 Apr;38(2):784-99
Proteomic analysis of human epithelial lining fluid by microfluidics-based nanoLC-MS/MS: a feasibility study.
Franciosi L, Govorukhina N, Fusetti F, Poolman B, Lodewijk ME, Timens W, Postma D, ten Hacken N, Bischoff R
Electrophoresis 2013 Sep;34(18):2683-94
Electrophoresis 2013 Sep;34(18):2683-94
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HMGB1/HMG-1 Antibody (1B11) [H00003146-M03] - Analysis of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody (M03), clone 1B11. Lane 1: HMGB1 transfected lysatE (25 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: HMGB1/HMG-1 Antibody (1B11) [H00003146-M03] - Detection limit for recombinant GST tagged HMGB1 is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1B11) [H00003146-M03] - Analysis of monoclonal antibody to HMGB1 on HeLa cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (1B11) [H00003146-M03] - Analysis of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml
