AF5947

antibody from Novus Biologicals

Targeting: TLE1 ESG, ESG1, GRG1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5947

Provider

Novus Biologicals

Product name

Sheep Polyclonal TLE1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human TLE1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TLE3 and rhTLE4 is observed and less than 5% cross-reactivity with rhTLE2 is observed.

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human TLE1 by Western Blot. Western blot shows lysates of human muscle tissue and human heart tissue. PVDF Membrane was probed with 2 µg/mL of Human TLE1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5947) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TLE1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.