Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- TA319192 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal alpha-Tubulin antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal alpha-Tubulin antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- TUBA1B
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 200 µg
- Concentration
- 1.12 mg/mL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Western Blot of Rabbit Anti-Alpha Tubulin Antibody. Lane 1: whole cell lysates from mouse brain. Lane 2: rat brain. Lane 3: A431 cells. Lane 4: Jurkat cells. Lane 5: HeLa cells. Load: 35 ug per lane. Primary antibody: Alpha Tubulin antibody at 1:1,200 for overnight at 4°C. Secondary antibody: IRDye800? rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~50 kDa corresponding to alpha tubulin (arrowhead). Other band(s): none.
- Validation comment
- WB