PA5-63604

antibody from Invitrogen Antibodies

Targeting: HNRNPU C1orf199, FLJ30202, FLJ37978, HNRNPU-AS1, HNRPU, NCRNA00201, SAF-A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-63604

Provider

Invitrogen Antibodies

Product name

hnRNP U Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: AVLKMKGNFT LPEVAECFDE ITYVELQKEE AQKLLEQYKE ESKKALPPEK KQNTGSKKSN KNKSGKNQFN RGGGHRGRGG FNMRGG

Concentration

0.05 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of hnRNP U in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a hnRNP U Polyclonal Antibody (Product # PA5-63604). Remaining relative intensity is presented.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-hnRNP U Polyclonal Antibody (Product # PA5-63604) and a 110 kDa band corresponding to hnRNP U was observed in HeLa, Jurkat, NETRA-2 cl.D1, K-562 and was alos found to increase in THP-1 differentiated to macrophage upon treatment with Lipopolysaccharide in comparison to THP-1 differentiated to macrophage. Whole cell extracts (30 µg) of THP-1 differentiated to macrophage (Lane 1), THP-1 differentiated to macrophage and treated with lipopolysaccharide (100 ng/mL for 6 hours) (Lane 2), HeLa (Lane 3), Jurkat (Lane 4), NTERA-2 cl.D1 (Lane 5) and K-562 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of hnRNP U in human cell line Hep G2 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a hnRNP U Polyclonal Antibody (Product # PA5-63604).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of hnRNP U was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with hnRNP U Rabbit Polyclonal Antibody (Product # PA5-63604) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification..

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of hnRNP U in human cerebral cortex tissue shows strong nuclear positivity in neuronal cells, glial cells and endothelial cells, neuropil is negative. Samples were probed using a hnRNP U Polyclonal Antibody (Product # PA5-63604).