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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 700565 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-AS160 (Thr642) Recombinant Rabbit Monoclonal Antibody (10H18L7)
- Antibody type
- Other
- Antigen
- Synthetic peptide
- Description
- Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 10H18L7
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Phospho-AS160 pThr642 in whole cell extract (80 µg per lane) from HeLa cells (serum-starved) treated with IGF1 (150 ng/mL, 15 min) using a Phospho-AS160 pThr642 recombinant rabbit monoclonal antibody (Product # 700565) at a dilution of 2.5 µg/mL. Detection was performed using an HRP-conjugated goat anti-rabbit secondary antibody at a dilution of 1:5000 followed by chemiluminescence (ECL). Results show a band at ~160 kDa.
