Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- NBP1-78763 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-78763, RRID:AB_11004677
- Product name
- Rabbit Polyclonal IRF1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: IRF1 Antibody [NBP1-78763] - Whole cell lysate from NIH3T3 (M; 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-IRF1 antibody Aused for WB at 0.2 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IRF1 was also immunoprecipitated by rabbit anti-IRF1 antibodies NBP1-78761 and NBP1-78762, which recognize disparate epitopes.