LS-C750565

antibody from LSBio

Targeting: EIF2S1 EIF-2alpha, EIF2, EIF2A

Western blot (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C750565

Provider

LSBio

Product name

EIF2S1 Antibody (phospho-Ser51) LS-C750565

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-eIF2α-S51 pAb. Western blot was performed from the immunoprecipitate using Phospho-eIF2α-S51 pAb at a dilition of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-eIF2α-S51 pAb. Western blot was performed from the immunoprecipitate using Phospho-eIF2α-S51 pAb at a dilition of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.