- PA5-21810 - Invitrogen Antibodies SUCLG2 antibody

No submitted references: Submit reference

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using SUCLG2 Polyclonal Antibody (Product # PA5-21810). Sample (30 µg whole cell lysate). A: 293T. B: A431. C: H1299. D: HeLa S3. E: HepG2. F: MOLT4. G: Raji. 10% SDS PAGE. SUCLG2 Polyclonal Antibody (Product # PA5-21810) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using SUCLG2 Polyclonal Antibody (Product # PA5-21810). Sample (20 µg of whole cell lysate). Lane A: mouse liver. 10% SDS PAGE. SUCLG2 Polyclonal Antibody (Product # PA5-21810) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of SUCLG2 was achieved by transfecting Hep G2 with SUCLG2 specific siRNAs (Silencer® select Products # s16774, s16775). Western blot analysis (Fig. a) was performed using whole cell extracts from the SUCLG2 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with SUCLG2 Polyclonal Antibody (Product # PA5-21810, 1:2000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25ug/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SUCLG2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-SUCLG2 Polyclonal Antibody (Product # PA5-21810) and a 47kDa band corresponding to SUCLG2 was observed in all the cell lines and tissues tested. Whole cell extracts (30ug lysate) of Hep G2 (Lane 1), MOLT-4 (Lane 2), Raji (Lane 3), A549 (Lane 4), U-2 OS (Lane 5), MCF-7 (Lane 6), tissue extracts (30ug lysate) of Mouse Liver (Lane 7), Mouse Kidney (Lane 8), Mouse Brain (Lane 9) and Mouse Ovary (Lane 10) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of SUCLG2 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: SUCLG2 Polyclonal Antibody (Product # PA5-21810) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SUCLG2 Polyclonal Antibody detects SUCLG2 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. SUCLG2 Polyclonal Antibody (Product # PA5-21810) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PA5-21810