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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA1-28383 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-NF2 (Ser518) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-28383 detects NF2 from mouse, human samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Affinity purified phospho-specific antibody to NF2 (Merlin) at pS518 Phospho-NF2 (Ser518) Polyclonal Antibody (Product # PA1-28383) was used at a 1:1,000 dilution to detect NF2 by Western blot. Approximately 12 µL of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4 nm, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (Product # PA1-28773) preceded color development using Amershams substrate system. Other detection methods will yield similar results.
