TA590014

antibody from OriGene

Targeting: NF2 ACN, BANF, merlin, SCH

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA590014

Provider

OriGene

Product name

Rabbit Polyclonal Merlin Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal Merlin Antibody

Reactivity

Canine

Host

Rabbit

Conjugate

Unconjugated

Epitope

NF2

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

0.76mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot: Neurofibromin 2 Antibody - Western Blot was performed using affinity purified SEQer NF2 antibody, aa(477-576) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer NF2 antibody, aa(477-576) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NF2 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

ELISA

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

ELISA: Neurofibromin 2 Antibody

Validation comment

ELISA

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using anti-NF2 rabbit polyclonal antibody (TA590014).

Validation comment

IF