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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-71839 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-71839, RRID:AB_11017663
- Product name
- Rabbit Polyclonal PA2G4/EBP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PA2G4/EBP1 Antibody [NBP1-71839] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. EBP1 was more efficiently immunoprecipitated by rabbit anti-EBP1 antibody NBP1-71840, which recognizes a downstream epitope.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody [NBP1-71839] - FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EBP1, left image and , right image used at a dilution of 1:200 (1 ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).
