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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX60840 - Provider product page
- Provider
- GeneTex
- Product name
- RPB1 (phospho Ser5) antibody [GT9082]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- WB analysis of nuclear extracts (25 ?g) from HeLa cells using Pol II (phospho Ser2) antibody at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of nuclear extracts (25 £gg) from HeLa cells using Pol II (phospho Ser2) antibody at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of HeLa cells using Pol II (phospho Ser2) antibody (red) and DAPI (blue). Cells were fixed with methanol and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with Pol II (phospho Ser2) antibody at a dilution of 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ChIP was performed with HeLa extracts and either control IgG (2 £gg) or Pol II (phospho Ser5) antibody (1, 2, 5 and 10 £gg per ChIP experiment). The precipitated DNA was detected by PCR with primers targeting to positive controls the promoter and the coding region of GAPDH and ACTB genes, and to negative controls the exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat.
