MA5-18089

antibody from Invitrogen Antibodies

Targeting: POLR2A POLR2, POLRA, RPB1

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Recommended by provider)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA5-18089

Provider

Invitrogen Antibodies

Product name

Anti-Phospho-POLR2A (Ser5) Monoclonal Antibody (4H8)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody recognizes the C-terminal repeat of the largest subunit of RNA polymerase II, both unphosphorylated and phosphorylated forms, in mouse, human, S. cerevisiae, A. thaliana, C. elegans, Drosophila, S. pombe and African Green monkey samples.

Reactivity

Human, Mouse, Drosophila, Yeast

Host

Mouse

Isotype

IgG

Antibody clone number

4H8

Vial size

100 µL

Concentration

0.8 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RNA polymerase II in HeLa (left) and MCF7 cells (right) using a Phospho-RNA polymerase II (Ser5) monoclonal antibody (MA5-18089).