NBP2-50243

antibody from Novus Biologicals

Targeting: POLR2A POLR2, POLRA, RPB1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

NBP2-50243

Provider

Novus Biologicals

Product name

Mouse Monoclonal RNA Polymerase II/POLR2A Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human, Yeast

Host

Mouse

Isotype

IgM

Vial size

0.1 ml

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RNA Polymerase II/POLR2A Antibody (CTD 8A7) [NBP2-50243] - RNA polymerase II (RNAP II) contains 12 subunits. The C-terminal domain of the largest subunit of RNAP II is reversibly phosphorylated on the highly conserved heptapeptide repeat, YSPTSPS. The major physiological phosphorylation sites are Ser-2 and Ser-5. Monoclonal antibody 8A7 was obtained using synthetic phosphopeptides and specifically recognises the Ser-5 phosphorylated form of RNAP II.