Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- PA5-28826 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Syntenin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: U87-MG.
- Concentration
- 0.78 mg/mL
Submitted references Molecular Profiling and Functional Analysis of Macrophage-Derived Tumor Extracellular Vesicles.
Cianciaruso C, Beltraminelli T, Duval F, Nassiri S, Hamelin R, Mozes A, Gallart-Ayala H, Ceada Torres G, Torchia B, Ries CH, Ivanisevic J, De Palma M
Cell reports 2019 Jun 4;27(10):3062-3080.e11
Cell reports 2019 Jun 4;27(10):3062-3080.e11
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Syntenin 1 Polyclonal Antibody (Product # PA5-28826). HeLa whole cell extracts and HeLa exosome extract (3.5 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 Polyclonal Antibody (Product # PA5-28826) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Syntenin 1 antibody detects Syntenin 1 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody Syntenin 1 Polyclonal Antibody (Product # PA5-28826) diluted by 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Syntenin 1 in methanol-fixed HeLa cells using a Syntenin 1 polyclonal antibody (Product # PA5-28826) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of Syntenin-1 was performed in frozen sectioned adult mouse brain using Syntenin 1 Polyclonal Antibody (Product # PA5-28826) at a dilution of 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of Syntenin-1 was performed in frozen sectioned adult mouse brain using Syntenin 1 Polyclonal Antibody (Product # PA5-28826) at a dilution of 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Syntenin 1 Polyclonal Antibody detects Syntenin 1 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Syntenin 1 Polyclonal Antibody (Product # PA5-28826) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Syntenin 1 Polyclonal Antibody detects Syntenin 1 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Syntenin 1 Polyclonal Antibody (Product # PA5-28826) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Syntenin 1 Polyclonal Antibody detects Syntenin 1 protein at cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Syntenin 1 Polyclonal Antibody (Product # PA5-28826) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.